Table 1.

Primer sequences and PCR conditions

GenePrimer sequenceCycling conditions (°C, s)
AnnealingElongationAcquisition
VEGF5′-TCTTCAAGCCATCCTGTGTG-3′ (forward); 5′-TCTCTCCTATGTGCTGGCCT-3′ (reverse)62, 1072, 1083, 5
HIF-1α5′-GGACAAGTCACCACAGGACA-3′ (forward); 5′-GGGAGAAAATCAAGTCGTGC-3 (reverse)62, 1072, 1079, 5
ET-15′-CCATGAGAAACAGCGTCAAA-3′ (forward); 5′-AGTCAGGAACCAGCAGAGGA-3′ (reverse)62, 1072, 1079, 5
Glut-15′-GGGCATGTGCTTCCAGTATGT-3′ (forward); 5′-ACCAGGAGCACAGTGAAGAT-3′ (reverse)62, 1072, 1079, 5
Niemann-Pick5′-TGGGCGCGATATTTCTGGTG-3′ (forward); 5′-CTCCACGCGGCTGCCTTTC-3′ (reverse)68, 572, 987, 5