Table 2.

TRAIL-mediated apoptosis induced by several drugs in Ramos and M202 melanoma cells

Apoptosis (%)
P*DR5 expressionYY1 expression
TRAIL (ng/mL)
010
RamosUntreated6.3 ± 0.916.1 ± 0.4231.5 ± 29.6633.6 ± 35
CDDP (1 μg/mL)12 ± 1.142.3 ± 1.10.02550.2 ± 18.7567.2 ± 41.4
CDDP (2 μg/mL)18.1 ± 0.447.6 ± 1.70.021602.4 ± 41.5374 ± 62.4
P0.010.0180.026
Untreated6.3 ± 0.916.1 ± 2.2231.5 ± 29.6633.6 ± 34.9
ADR (0.1 μg/mL)10.1 ± 1.236.4 ± 1.70.021350 ± 36.3539.7 ± 22
ADR (0.5 μg/mL)14.2 ± 1.442.2 ± 2.740.021464.5 ± 41420 ± 39.7
P0.0140.0170.020
M202Untreated8.3 ± 1.210.3 ± 0.9170.9 ± 11.8540.1 ± 25
VP-16 (5 μg/mL)15.6 ± 0.847.3 ± 3.20.02340.4 ± 39.3227.88 ± 40.8
VP-16 (15 μg/mL)21.3 ± 1.548.6 ± 2.10.02420.2 ± 80.1123.44 ± 20.7
P0.0180.0230.012
Untreated8.3 ± 1.210.3 ± 0.9170.9 ± 11.8540.1 ± 25
CDDP (1 μg/mL)8.9 ± 1.115.8 ± 1.60.035241.96 ± 25.1446.25 ± 35.3
CDDP (5 μg/mL)12.7 ± 0.719.2 ± 0.80.04255 ± 4401.22 ± 37.5
P0.0190.0450.019
Untreated8.3 ± 1.210.3 ± 0.9170.9 ± 11.8540.1 ± 2
Vincristine (0.01 μg/mL)11.2 ± 1.723 ± 1.70.021265 ± 24.7386.64 ± 24.5
Vincristine (0.1 μg/mL)15.8 ± 2.228.5 ± 2.20.025281.77 ± 40.9312.39 ± 32.8
P0.0240.0360.015
  • NOTE: Treatment of Ramos and M202 with the various drugs and TRAIL was done as described in Materials and Methods for PC-3. In addition, DR5 surface expression and intracytoplasmic YY1 protein determination were done following treatment for 18 h by flow cytometry. Apoptosis was determined by active caspase-3 or propidium iodide–based detection methods as described in Materials and Methods. Values represent either mean arbitrary units ± SE for apoptosis determination or mean fluorescence intensity ± SEM for protein expression.

  • * Kruskal-Wallis H test, degrees of freedom = 2.