Table 1.

Effects of R16 and amonafide on the MDR sublines

CompoundsIC50 (mean ± SD) (μM)
RFIC50 (mean ± SD) (μM)
RFIC50 (mean ± SD) (μM)
RF
K_562K_562/A02KBKB/VCRMCF_7MCF_7/ADR
R162.43 ± 0.942.07 ± 0.350.851.25 ± 0.903.31 ± 0.41*2.644.30 ± 2.296.07 ± 1.611.41
Am6.81 ± 2.656.34 ± 0.380.932.86 ± 0.348.46 ± 0.732.969.24 ± 2.779.24 ± 3.411.00
VP165.12 ± 0.94258.81 ± 84.5250.590.45 ± 0.08163.05 ± 76.93300.9915.44 ± 5.16487.41 ± 40.23*31.57
ADR0.40 ± 0.1946.57 ± 11.42117.01NTNTNT1.55 ± 0.77150.30 ± 68.65*96.86
VCRNTNTNT3.11 ± 1.560.61 ± 0.03197.39NTNTNT
  • NOTE: Three separate experiments were carried out to determine the values of IC50, expressed as mean ± SD (μmol/L), and the inhibition rates of each concentration of compounds were tested in triplicate. The resistance factor was calculated as the ratio of the IC50 value of the MDR cells to that of the corresponding senaltive parental cells. The significance of IC50 values of each compound against MDR cells and corresponding parental cells was analyzed and indicated.

  • * P < 0.05.

  • P < 0.01.

  • The IC50 of VCR against KB cells was 3.11 ± 1.56 nmol/L.