Table 1.

mRNA expression of BLyS, BAFF-R, TACI, BCMA and comparative IC50 values of the rGel/BLyS fusion toxin against various types of tumor cell lines

Cell lineCell typeBLyS (313 bp)BAFF-R (256 bp)TACI (196 bp)BCMA (285 bp)IC50 (nmol/L)
Targeting index*
rGelrGel/BLyS
BCL-1Mouse B lymphomaNDNDNDND1500.0008187,500
JeKo-1MCL+++++++++4000.00580,000
MinoMCL+++++++700.00235,000
SP53MCL++++++++++1100.00427,500
Granta 519MCL++++++++1,0001506.7
OCI-Ly3DLBCLNDNDNDND100.00110,000
SUDHL-6DLBCLNDNDNDND7005140
RPMI 8226Plasmacytoma myeloma+++++++2801028
8226/LR-5Plasmacytoma myeloma+++++++2001101.8
IM-9Multiple myeloma++++++++7002003.5
MM1.SMultiple myeloma++++++6002202.7
MM1.RMultiple myeloma++++++1,0002803.6
HL-60Acute promyelocytic leukemia+++1,0003003.3
KBM-5Myeloid leukemia++250703.6
THP-1Acute monocytic leukemia++++110303.7
JurkatAcute T-cell leukemia+++3,0001,5002.0
  • NOTE: The mRNA levels were normalized to GAPDH. +, low expression (relative density = 0.3–0.7); ++, intermediate expression (relative density = 0.8–1.3); +++, high expression (relative density >1.4).

  • Abbreviation: ND, not determined.

  • *Targeting index represents IC50 of rGel / IC50 of rGel/BLyS.