Table 3.

Cytokine production data

Cat no.Dose level (pfu/tumor)Tumor volume (cm3)Injection sitesIL-12 mRNA (relative fold increase over baseline, 24 h post-HT)IFN-γ mRNA (relative fold increase over baseline, 24-48 h post-HT)
1109130.0382.7 ± 0.3Trace (+/−)*
21091.1219,000 ± 5,233Trace (+/−)
310926.01597 ± 76.2 ± 0.9
410102.523,028 ± 114Trace (+/−)
510101.621,685 ± 184Trace (+/−)
6101012.416358 ± 30Trace (+/−)
710102.323,841 ± 643Trace (+/−)
84 × 10105.0101,318 ± 31242 ± 20
94 × 101031.7277,043 ± 11451.7 ± 0.2
104 × 10101.5529,565 ± 9,208Trace (+/−)
114 × 101037.02014,658 ± 2,055152 ± 34
124 × 101028.8252,793 ± 4896.2 ± 2.5
1310101.941,858 ± 691Trace (+/−)
  • NOTE: Details of the tumor volumes and number of injections for the cats treated at various dose levels are shown. The IL-12 and IFN-γ mRNA levels were measured using RT-PCR in the tumors at 24 h and 24 to 48 h post-hyperthermia (post-HT) respectively.

  • * IFN-γ production detectable in some PCR wells as increase in the real-time amplification curve, but not unequivocal.

  • Forty-eight hours post-hyperthermia; only one extra tumor biopsy was possible due to small tumor size.

  • Cat febrile after gene injection on Monday for ∼12 h.