Table 2.

Effects of XK469 and SH80 on DEVDase activation

ConditionsDEVDase (nmol/min/mg protein)
60 min24 h48 h
Control0.08 ± 0.030.12 ± 0.060.15 ± 0.04
HA14-14.42 ± 0.48
XK4720.12 ± 0.050.15 ± 0.07
XK4690.18 ± 0.070.17 ± 0.05
SH800.20 ± 0.050.22 ± 0.07
XK472/HA14-14.13 ± 0.403.28 ± 0.47
XK469/HA14-13.98 ± 0.521.38 ± 0.37
SH80/HA14-14.10 ± 0.472.90 ± 0.30
  • NOTE: In the first five treatment rows, cultures were treated with nothing, 40 μmol/L of HA14-1, or 50 μmol/L of XK472, or CI90 concentrations of XK469 or SH80 for the indicated times prior to being harvested. In the bottom three rows, cultures were exposed to 50 μmol/L of XK472 or CI90 concentrations of XK469 or SH80 for the indicated times plus HA14-1 during the last hour of incubation. Data represents the results of three experiments (mean ± SD).