Table 1.

Effects of XK469 analogues, wortmannin, and Atg7 deficiency on cell cycle progression

TreatmentElapsed time (h)Cells in designated phase (%)
G0-G1SG2-M
Experiment 1
    Solvent24474013
48493516
    XK47224543114
48533215
    XK46924103159
48112366
    SH8024142759
48162460
Experiment 2
    Solvent24355510
48385111
72434710
    Wortmannin24365311
48375112
72474210
    XK46924104050
4812772
7252372
    XK469 + wortmannin2485042
4833760
7242472
Experiment 3
    WT-L121048405010
    WT-L1210 + XK4692434057
4821880
7232473
    Atg7-L1210 + XK4692462965
4831879
7262173
  • NOTE: In experiment no. 1, L1210 cultures were treated with solvent, 50 μmol/L of XK472 or CI90 concentrations of XK469 or SH80 for the indicated amounts of time prior to being harvested for analysis of DNA contents by flow cytometry. In experiment no. 2, L1210 cultures were treated with solvent, 100 nmol/L of wortmannin, a CI90 concentration of XK469 or XK469 + wortmannin for the indicated lengths of time before harvesting. In experiment no. 3, wild-type or Atg7-deficient L1210 cultures were treated with a CI90 concentration of XK469 for the indicated lengths of time before harvesting.