Table 2.

Growth inhibition of D501036 against KB-derived cancer cell lines with different resistance phenotypes

KB-derived cell linesResistance typeIC50 (nmol/L)
D501036VincristinePaclitaxelVP16
KBParental466 ± 800.6 ± 0.24.1 ± 1.61.1 ± 0.2
KBvin10MDR ↑362 ± 9390.1 ± 7.416,500 ± 70723 ± 3.0
KBtax50MDR ↑323 ± 871.8 ± 0.5273 ± 153.5 ± 0.3
KB-7DTopoisomerase II ↓ / MDR ↑3,200 ± 3801.2 ± 0.47.9 ± 0.554 ± 3.5
  • NOTE: Cells were treated with various concentrations of the test compounds for 72 h. Cell survival was determined by 3-(4,5-dimethythiazol-2-yl)-5-(3-carbomethoyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Each IC50 value was calculated as described in Materials and Methods. Each value represents the mean ± SD of three independent experiments.