Table 1.

Distribution of 5-methylTHF- and methotrexate polyglutamates as a fraction of total intracellular 5-CH3-[3H]-THF and [3H]-methotrexate polyglutamates

(A) Effects of FPGS overexpression on 5-methylTHF- and methotrexate polyglutamate distribution
5CH3-THF-polyglutamate distribution (% of total 5CH3-THF)
Glu1Glu2Glu3Glu4Glu5Glu6Glu7Glu8
Sense000014403412
Control-S00032653170
Methotrexate-polyglutamate distribution (% of total methotrexate)

Glu1
Glu2
Glu3
Glu4
Glu5
Glu6


Sense521125552
Control-S511521950
(B) Effects of FPGS inhibition on 5-methylTHF- and methotrexate polyglutamate distribution
5CH3-THF-polyglutamate distribution (% of total 5CH3-THF)

Glu1
Glu2
Glu3
Glu4
Glu5
Glu6
Glu7
Glu8
Control-si0000353396
siRNA0001524430
Methotrexate-polyglutamate distribution (% of total methotrexate)

Glu1
Glu2
Glu3
Glu4
Glu5
Glu6


Control-si18172814220
siRNA602016400