Table 3.

Apoptosis of endothelial cells in xenografts treated with AS Bcl-2 ODN alone, irradiation alone, or in combination after s.c. implantation of human prostate PC-3-Bcl-2 cancer cells

Treatment groupApoptotic index for endothelial cells*
Mock no XRT0.26 ± 0.07
Mock + XRT0.24 ± 0.09
CTL no XRT0.53 ± 0.20
CTL + XRT0.32 ± 0.17
AS Bcl-2 ODN no XRT1.24 ± 0.45
AS Bcl-2 ODN + XRT3.81 ± 0.77
  • * The apoptotic index for endothelial cells of microvessels (as measured by immunofluorescent double staining) is expressed as the average immunofluorescence of apoptotic cells (TUNEL assay) against all endothelial cells (CD31 immunofluorescence) present in the five areas of highest microvessel density identified within a single ×200 field ± SD.