Table 1.

Effect of apigenin on cell cycle distribution in 22Rv1 and PC-3 cells

TreatmentsG0-G1 phaseG2-M phaseS phaseSub-G1 phase
22Rv1 cells
    Vehicle only54.7 ± 2.4 (—)15.8 ± 1.8 (—)29.5 ± 1.6 (—)2.0 ± 0.8 (—)
    10 μmol/L apigenin59.4 ± 2.1 (0.024)15.9 ± 1.4 (NS)24.7 ± 1.3 (0.005)7.9 ± 1.1 (0.0005)
    20 μmol/L apigenin61.3 ± 1.8 (0.008)16.3 ± 1.6 (NS)22.4 ± 1.2 (0.002)9.5 ± 1.3 (0.0002)
    40 μmol/L apigenin62.1 ± 2.0 (0.004)16.2 ± 1.5 (NS)21.7 ± 1.5 (0.001)11.3 ± 1.2 (0.0001)
    80 μmol/L apigenin65.0 ± 1.7 (0.003)17.8 ± 1.2 (NS)17.2 ± 1.4 (0.0001)32.3 ± 1.6 (<0.0001)
PC-3 cells
    Vehicle only47.6 ± 1.4 (—)30.2 ± 1.4 (—)22.2 ± 1.1 (—)0.4 ± 0.2 (—)
    10 μmol/L apigenin51.4 ± 1.5 (0.005)24.5 ± 1.2 (0.008)24.1 ± 1.5 (NS)1.2 ± 0.5 (0.0034)
    20 μmol/L apigenin57.4 ± 1.2 (0.0002)24.6 ± 1.3 (0.008)18.0 ± 1.5 (0.03)5.2 ± 0.8 (0.007)
    40 μmol/L apigenin60.8 ± 1.8 (0.0003)22.5 ± 1.6 (0.003)16.7 ± 1.2 (0.004)10.1 ± 1.0 (0.002)
    80 μmol/L apigenin62.6 ± 1.3 (<0.0001)17.2 ± 1.4 (0.0005)20.2 ± 1.7 (NS)18.4 ± 2.2 (0.0008)
  • NOTE: The cells were treated with vehicle alone or with indicated doses of apigenin for 24 hours, stained with propidium iodide, and analyzed by flow cytometry. Percentage of cells in sub-G1, G0-G1, S, and G2-M phases were calculated using cell-fit computer software. Each value represents the mean ± SD from three independent experiments. Values are represented as percentage of cell population. Values in parens represent P values.

  • Abbreviation: NS, not significant.