Table 1.

Effect of broccoli sprout isothiocyanate extract on cell cycle progression of UM-UC-3 cells

Isothiocyanate extract (μmol/L)Cell cycle distribution (%)
Sub-G1G1SG2-MCells with >4n DNA
24-h treatment
    02.1 ± 0.453.3 ± 1.732.9 ± 1.610.9 ± 0.40.8 ± 0.2
    7.52.2 ± 0.544.1 ± 0.737.6 ± 0.8*14.4 ± 0.5*1.6 ± 0.2
    153.4 ± 0.812.1 ± 0.442.5 ± 2.4*38.7 ± 0.9*3.4 ± 1.4*
    303.3 ± 1.047.0 ± 0.131.3 ± 1.217.0 ± 0.7*1.4 ± 0.1
48-h treatment
    02.1 ± 0.279.0 ± 0.811.8 ± 0.56.0 ± 0.51.1 ± 0.2
    7.55.7 ± 0.7*61.2 ± 1.222.7 ± 0.7*8.9 ± 1.0*1.6 ± 0.8
    155.6 ± 1.2*33.4 ± 0.315.5 ± 0.5*30.7 ± 0.9*14.8 ± 1.0*
    304.7 ± 1.0*44.8 ± 2.125.9 ± 0.8*23.0 ± 0.7*1.6 ± 0.6
72-h treatment
    05.1 ± 1.283.8 ± 2.96.3 ± 0.43.8 ± 1.81.0 ± 0.4
    7.510.0 ± 1.2*71.7 ± 1.513.0 ± 0.5*3.8 ± 0.91.5 ± 0.1
    1514.4 ± 1.5*36.8 ± 4.314.6 ± 0.1*20.7 ± 2.1*13.5 ± 3.1*
    305.7 ± 0.739.7 ± 2.728.6 ± 1.6*24.2 ± 1.7*1.8 ± 0.1
  • NOTE: Cells were grown in 10 cm plates (1.5 × 106 per plate with 10 mL medium) for 24 hours and then exposed to isothiocyanate extract at desired concentrations for 24, 48, or 72 hours, respectively. Cells were harvested and analyzed by flow cytometry. Values are mean ± SD, n = 3.

  • * P < 0.05, different from the control.