Table 1.

Flow cytometric analysis of Notch-1-transfected cells

G0-G1 (%)S (%)G2-M (%)
BxPC-3
    CS55.8 ± 0.730.6 ± 1.113.6 ± 0.9
    NS72.4 ± 1.422.1 ± 1.55.5 ± 0.6
    CP52.3 ± 3.529.2 ± 3.118.5 ± 0.4
    NP37.8 ± 1.242.4 ± 2.019.8 ± 3.1
HPAC
    CS46.9 ± 2.436.3 ± 1.516.8 ± 1.2
    NS66.2 ± 1.824.7 ± 1.59.1 ± 1.1
    CP53.8 ± 2.034.4 ± 1.311.8 ± 2.1
    NP40.1 ± 1.936.8 ± 1.523.1 ± 1.6
PANC-1
    CS40.5 ± 2.433.7 ± 1.825.8 ± 1.6
    NS67.4 ± 3.515.3 ± 1.217.3 ± 2.8
    CP46.1 ± 2.135.5 ± 1.518.4 ± 0.9
    NP29.8 ± 2.654.3 ± 1.215.9 ± 1.7
  • NOTE: Flow cytometry was done as described in Fig. 4. The mean values (± SE) represent the percentage of cells in the indicated phase of the cell cycle from three independent experiments.

    Abbreviations: CS, control siRNA; NS, Notch-1 siRNA; CP, control plasmid; NP, Notch-1 plasmid.