Flow cytometric analysis of Notch-1-transfected cells
| G0-G1 (%) | S (%) | G2-M (%) | ||||
|---|---|---|---|---|---|---|
| BxPC-3 | ||||||
| CS | 55.8 ± 0.7 | 30.6 ± 1.1 | 13.6 ± 0.9 | |||
| NS | 72.4 ± 1.4 | 22.1 ± 1.5 | 5.5 ± 0.6 | |||
| CP | 52.3 ± 3.5 | 29.2 ± 3.1 | 18.5 ± 0.4 | |||
| NP | 37.8 ± 1.2 | 42.4 ± 2.0 | 19.8 ± 3.1 | |||
| HPAC | ||||||
| CS | 46.9 ± 2.4 | 36.3 ± 1.5 | 16.8 ± 1.2 | |||
| NS | 66.2 ± 1.8 | 24.7 ± 1.5 | 9.1 ± 1.1 | |||
| CP | 53.8 ± 2.0 | 34.4 ± 1.3 | 11.8 ± 2.1 | |||
| NP | 40.1 ± 1.9 | 36.8 ± 1.5 | 23.1 ± 1.6 | |||
| PANC-1 | ||||||
| CS | 40.5 ± 2.4 | 33.7 ± 1.8 | 25.8 ± 1.6 | |||
| NS | 67.4 ± 3.5 | 15.3 ± 1.2 | 17.3 ± 2.8 | |||
| CP | 46.1 ± 2.1 | 35.5 ± 1.5 | 18.4 ± 0.9 | |||
| NP | 29.8 ± 2.6 | 54.3 ± 1.2 | 15.9 ± 1.7 | |||
NOTE: Flow cytometry was done as described in Fig. 4. The mean values (± SE) represent the percentage of cells in the indicated phase of the cell cycle from three independent experiments.
Abbreviations: CS, control siRNA; NS, Notch-1 siRNA; CP, control plasmid; NP, Notch-1 plasmid.