Table 3.

Cytotoxicity of 5HPP-33, paclitaxel, and colchicine on wild-type and MDR transfected NIH3T3 cells

DrugNIH3T3
NIH3T3-G185
NIH3T3-V185
IC50 (μmol/L)RRIC50 (μmol/L)RRIC50 (μmol/L)RR
5HPP-33313.61.24.51.5
Paclitaxel0.0412.2857125
Colchicine0.0610.46.75.287
  • NOTE: Cytotoxicity of 5HPP-33, paclitaxel, and colchicine on NIH3T3 cells alone or transfected with retroviral vectors expressing either of two forms of MDR1: wild-type (G185) or a point mutant (V185) that alters MDR selectivity. Average of two separate experiments. RR = (ratio of the IC50 of transfected cells) / (IC50 of the nontransfected cells).