Table 2.

Killing of HeLa cells by Y6 reversion enzymes

VariantMMC viability (%)CB 1954 viability (%)
Cells100 ± 8100 ± 5
YieF65 ± 972 ± 2
Y626 ± 37 ± 3
A120V24 ± 44 ± 5
N128Y95 ± 3102 ± 8
N160T30 ± 912 ± 2
L175Q26 ± 29 ± 4
  • NOTE: HeLa cells were incubated in the presence of 15 μmol/L of one of the prodrugs and 50 μg/mL of the enzyme.

    Abbreviation: MMC, mitomycin C.