Table 1.

Bacterial strains, plasmids, genes, and primers

Relevant characteristicsReference or source
Strain
    E. coli BL21DE3 allowing overexpression of desired protein under IPTG-inducible T7 promoterNovagen
    S. typhimurium SL 7838Attenuated strain containing aroA and sopE gene deletionsThorne et al., unpublished data
Plasmids
    pET28a+pET28a+ expressing the desirable gene (NdeI/BamHI)(9)
    pCGLS1Containing Luciferase gene allowing in vivo imaging(16)
    pET28a+::yieF*E. coli YieF (Genbank accession no. NC_000913.2)(9)
    pET28a+::nfsA*E. coli NfsA (Genbank accession no. P17117)(24)
Primers
    FyieF5′-CGCGGGGGCATATGTCTGAAAAATTGCAGGT-3′(9, 12)
    RyieF5′-TTTGGGATCCTTAGATCTTAACTCGCTGAA-3′(9, 12)
    FA120V5′-GTATTGATTCAGACCAGCTCAATGGGCGTGATTGG-3′This research
    RA120V5′-CCAATCACGCCCATTGAGCTGGTCTGAATCAATAC-3′This research
    FN128Y5′-TTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGA-3′This research
    RN128Y5′-TCTGGCGCAGGTGATACTGACAGCGCGCGCCGCCAAT-3′This research
    FN160T5′-GTTGATCCGCAAACCGGAGAAGTGATTGA-3′This research
    RN160T5′-ATCAATCACTTCTCCGGTTTGCGGATCAAC-3′This research
    RL175G5′-TTAACTCGCTGAATAAACTCACCAAATGCGGTCAATTGCCCGGTCAGGTG-3′This research
  • Abbreviation: IPTG, isopropyl-β-d-thiogalactopyranoside.

  • * Protein accession number in PubMed database.

  • Bold underlie NdeI restriction site.

  • Bold underlie BamHI restriction site.