Table 1.

Transcript copy number per microgram of total RNA of the different GR transcriptional isoforms and promoters expressed in MM.1S, MM.1Re, and MM.1RL cell lines

GRα*GRβ*GR-PGRγGR-APromoter APromoter BPromoter C
MM.1S1.1 × 1071.7 × 1034.9 × 1055.2 × 105No0.05.8 × 1054.8 × 106
MM.1Re2.6 × 1051.4 × 1023.8 × 1053.8 × 104Yes0.02.1 × 1051.6 × 106
MM.1RL1.8 × 1022.6 × 1011.6 × 1032.2 × 101No0.01.3 × 1058.3 × 105
  • NOTE: Results were obtained by one-step real-time RT-PCR. The lower limit of detection for transcript number is 10 copies.

  • * GRα and GRβ real-time quantitative results also quantify GRγ and GR-A transcripts.

  • GR-P quantitative results also include GRγ transcripts.