Table 3.

Characteristics of the eight main primers used for PCR

SNPsPrimers sequencesSenseTmAmplification length
IVS1 + 1G AATCAGTGAGAAAACGGCTGCSense60150
TAAACATTCACCAACTTATGCCAAntisense52
−1590T>CATCCAGGACTCTCCCCAGATSense59.4201
CCTTGGTCAGATCCCAGAAGAntisense59.4
2846A>TAAGCACTGCAGTACCTTGGAASense57.9101
TCATGTAGCATTTACCACAGTTGAAntisense57.6
85T>CCCTGGCTTTAAATCCTCGAASense55.398
TCTTATCAGGATTTCTTTTCCAATGAntisense56.4
1156G>TTGGAACTTGCTAAGGAAGAAAASense54.7124
AACCAAAGGCACTGATGACCAntisense57.3
2657G>ATCTGACCTAACATGCTTCSense51.4100
AGGCCTTTTGGGGATAAAACAntisense55.3
2983G>TTTTGATCCAGAAACCCACCTSense55.3139
AGGGTACGCCTCTCTTTGGTAntisense59.4
Del TCATTGTCAGAAGAGCTGTCCAASense54.562
TTGTTTGCAATACTTGTGATGAntisense52