Table 1.

Expression and binding of soluble Fabs selected as phage-displayed Fabs for their strong binding to precursor IGF-II

CloneExpressionBindingH3 sequence
m606++++++++DRSIAAMGWFDH
m610++++++++DVQWLAYGMDV
m616+++++EKGIGRGITGTTIPYNWFDP
m618
m622
m624
m626
m627+
m635
m641++
  • NOTE: Ten clones with unique sequences, which significantly bound to a precursor IGF-II form as phage-displayed Fabs, were expressed as free Fabs, purified and tested for binding to IGF-II in ELISA. The number of + symbols is a measure of their expression and binding activity.