Table 3.

Comparison of MGMT recovery kinetics following O6-benzylguanine treatment or recovery from methionine stress in CAPAN1 tumor cells

Time (h)O6-benzylguanine–treated MGMT (fmol/mg protein)Methionine stress treated
01,123 ± 55*34 ± 13
129 ± 134 ± 12
2464 ± 1529 ± 22
36464 ± 6535 ± 29
721,234 ± 91878 ± 113
  • * CAPAN1 cells grown in methionine medium and treated with 30 μmol/L O6-benzylguanine at time 0 for 6 hours, then washed thrice, and harvested at the time intervals indicated.

  • CAPAN1 cells under methionine stress for 6 days, then transferred into methionine medium at time 0, and harvested at the time intervals indicated. Average of six determinations ± SD.