Table 1.

Binding affinity of the bispecific diabody to KDR and Flt-4

AntibodyKDR binding
Flt-4 binding
kon (× 105 mol/L−1 s−1)koff (× 10−4 s−1)Kd (nmol/L)kon (× 105 mol/L−1 s−1)koff (× 10−4 s−1)Kd (nmol/L)
scFv 11213.4 ± 0.191.4 ± 0.010.41 ± 0.03NBNBNB
scFv 3C5NBNBNB15.6 ± 6.59.6 ± 0.10.71 ± 0.36
Diabody1.5 ± 0.40.88 ± 0.040.61 ± 0.195.3 ± 1.825.1 ± 6.15.0 ± 1.4
  • NOTE: All numbers are determined by BIAcore analysis and represent the mean ± SE of four to six separate determinations. Kd values are calculated as the ratios of koff/kon.

    Abbreviation: NB, no binding.