Table 2.

Treatment of H-RAS V12 (C10) cells with a FTI reduces plating efficiency and enhances radiosensitivity

Radiation dose (Gy)Vehicle (DMSO) treatmentFTI (2 μmol/L) treatment
01.00 ± 0.080.52 ± 0.07*
10.88 ± 0.070.21 ± 0.04
40.16 ± 0.040.02 ± 0.01
  • NOTE: H-RAS V12 (C10) cells were plated in parallel to those described in Fig. 4 legend and serum starved for 24 hours with simultaneous exposure to a FTI (FTI277, 2 μmol/L). Cells were subjected to a 1- or 4-Gy exposure or to a mock exposure, as described in Materials and Methods. Colonies counted 10 to 14 days later as described in Materials and Methods. The numbers of colonies were expressed as percentage of the respective mock exposed group (defined as 1.00). Data are the means of six separate dishes from three separate experiments ± SE.

  • * P < 0.05 plating efficiency less than corresponding vehicle-treated cell value.

  • P < 0.05 more radiosensitive than corresponding vehicle-treated value.