Table 2.

Specificity of Eg5 inhibitors tested on human Eg5 and other kinesins by measuring the inhibition of basal ATPase activity

NSC No.HsEg5* Eg52-386
Human Conventional Kinesin HK379A. nidulans BimC Trx_1-428Dimeric Ncd MC5
−Microtubules+Microtubules
5681725 ± 3.610.8 ± 3NI140 ± 30NI
1696766.6 ± 0.79.0 ± 1.4NININI
832651.0 ± 0.20.14 ± 0.01NININI
62212413 ± 3.5NINI28 ± 9
7820614 ± 136 ± 12NININI
11988988 ± 20NI115 ± 14NI
17203326.6 ± 6NININI
27071822 ± 7NININI
125034145 ± 50NININI
593495.6 ± 0.87.0 ± 2.2NININI
  • NOTE: The IC50 values are indicated in μmol/L. NI, not indicated.

  • * For human Eg5, the inhibition of the microtubule-activated ATPase activity is also shown.