Table 1.

Plasmid-based system for screening PMO sequence specificity and antisense activity

TransfectionTreatments% Luciferase Activity (Relative Luminescence Units/μg Protein)% Cell Viability
pCiNeoXIAP-lucΔAVehicle100.00 ± 1.93100.00 ± 2.72
Scrambled PMO115.00 ± 4.2393.78 ± 4.15
XIAP antisense53.78 ± 9.5369.30 ± 5.85
Vector controlVehicle100.00 ± 8.35100.00 ± 15.38
Scrambled PMO97.21 ± 3.9184.89 ± 9.50
LipofectAMINE aloneScrambled PMO0.006 ± 0.0183.00 ± 7.57
  • NOTE: HeLa cells transiently transfected with pCiNeoXIAP-lucΔA, vector alone (pCiNeo-lucΔA), or delivery agent (Lipofectamine) were scrape loaded with different agents as indicated. Luciferase activity was measured 24 hours later. Data are expressed as luciferase activity in lysates normalized for protein content and represent means ± SE of triplicate values (n = 2). Cell viability in each case was assessed by trypan blue staining and expressed as mean ± SE of triplicate values. P < 0.01, vehicle versus XIAP antisense PMO treated cells. P < 0.001, scrambled PMO versus XIAP antisense PMO treated cells transiently transfected with pCiNeoXIAP-lucΔA.