Table 2.

Induction of apoptosis by aminoflavone in aminoflavone-sensitive and aminoflavone-resistant cell lines

Cell LineAminoflavone (nmol/L)
00.11101001,000
MCF-70.3 ± 0.6*10.7 ± 3.114.3 ± 2.118.3 ± 4.725.3 ± 3.841.0 ± 2.0
AHR100000.7 ± 1.21.7 ± 2.11.7 ± 0.62.3 ± 1.5
MDA-MB-43500.3 ± 0.63.3 ± 1.55.0 ± 1.78.7 ± 3.28.7 ± 2.3
PC-3001.0 ± 01.0 ± 01.0 ± 1.72.3 ± 1.5
  • NOTE: Cell lines were treated with 0 to 1,000 nmol/L aminoflavone for 24 hours as described in Materials and Methods, stained with 4′,6-diamidino-2-phenylindole, and examined under a Leitz Diaplan fluorescent microscope. Apoptotic cells were defined by a nuclear shrinkage and chromatin condensation.

  • * Values are percentage apoptotic cells (mean ± SD) derived from examination of three separate fields each containing 100 cells.