Effects of PEITC and PITC on PC-3 cell cycle distribution
| Treatment | % Cells | ||||||
|---|---|---|---|---|---|---|---|
| Sub-G0-G1 | G0-G1 | S | G2-M | ||||
| Control (DMSO) | 4 ± 0.5 | 56 ± 1 | 15 ± 0.5 | 19 ± 0.5 | |||
| 5 μm PEITC | 14 ± 3* | 42 ± 3* | 14 ± 0.5 | 31 ± 1* | |||
| 10 μm PEITC | 16 ± 1* | 34 ± 5* | 13 ± 1 | 36 ± 4* | |||
| 5 μm PITC | 4 ± 0.5 | 59 ± 1 | 14 ± 0.5 | 20 ± 1 | |||
| 10 μm PITC | 4 ± 0.5 | 56 ± 0.5 | 15 ± 0.5 | 21 ± 0.5 | |||
Note: Cells were treated with DMSO (control) or different concentrations of PEITC or PITC for 24 h at 37°C. Both floating and attached cells were collected and processed for analysis of cell cycle distribution by flow cytometry following staining with propidium iodide. Data are means ± SE (n = 3).
↵* ,P < 0.05, significantly different compared with control (one-way ANOVA).