Table 1.

Drug concentrations (nm) required for 50% and 90% relative growth inhibition (IC50 and IC90)

Cell linesAdozelesin (nm)
Bizelesin (nm)
C-1027 (nm)
IC50IC90R2IC50IC90R2IC50IC90R2
HCT116WT0.271.20.980.997.90.982.08.10.98
HCT116p53−/−0.320.921.01.67.60.902.37.60.95
HCT116p21−/−0.050.751.00.485.30.900.887.00.95
  • Note: The indicated cell lines were treated with the drugs at various concentrations for 4 h and incubated for an additional 2 days in drug-free media. The number of viable cells relative to vehicle-treated control cultures was determined by XTT assay. The results of three independent experiments were averaged and the data were graphed versus the log drug concentration. The least squares method was used to derive the equation describing the line best fitting the data. The equation was used to estimate the IC50 and IC90 concentrations. The coefficient of determination (R2) is given for each data series.