Table 1.

Inhibitory activity of PD 0332991 against a panel of protein kinases

Protein kinaseIC50 (μmol/L)*
Cdk4/cyclin D10.011
Cdk4/cyclin D30.009
Cdk6/cyclin D20.015
Cdk2/cyclin E2>10
Cdk2/cyclin A>10
Cdk1/cyclin B>10
Epidermal growth factor receptor>10
Fibroblast growth factor receptor>10
Platelet-derived growth factor receptor>10
Insulin receptor>10
Lymphocyte kinase>10
Vascular endothelial growth factor receptor>10
AMP-activated protein kinase>10
Checkpoint kinase-1>10
Casein kinase-1>10
Casein kinase-2>10
c-Src kinase>10
C-terminal Src kinase>12
Dual-specificity tyrosine phosphorylation-regulated kinase 1A2.0
Glycogen synthase kinase-3β>10
c-Jun NH2-terminal kinase>10
Mitogen-activated protein kinase 2/Erk2>10
Mitogen-activated protein kinase–activated protein kinase 1a8.0
Mitogen-activated protein kinase–activated protein kinase 2>10
Mitogen-activated protein kinase kinase>10
Mitogen and stress-activated protein kinase 1>10
p70 Ribosomal protein S6 kinase>10
3-Phosphoinositide-dependent protein kinase 1>10
Phosphorylase kinase>10
Cyclic AMP-dependent protein kinase>10
Protein kinase B>10
Protein kinase C>10
p38-Regulated/activated kinase>10
Rho-dependent protein kinase>10
Stress-activated protein kinase 2a>10
Stress-activated protein kinase 3>10
Stress-activated protein kinase 4>10
Serum and glucocorticoid induced kinase>10
  • * Concentration of PD 0332991 necessary to inhibit activity by 50%. Mean of at least two separate determinations.