Table 2

Summary adduct Tm and half-life (37°C) determinations

Tm and half-life values for Adriamycin adducts were determined using data from Figs. 6 and 7. Melting profiles and half-lives were estimated by least squares fitting to sigmoidal and linear decays, respectively, and the SE of fit is shown.

TreatmentTm (14C)Tm (Southern)T½ (14C)T½ (Southern)
Adr/formaldehyde (cell free)72.2°C ± 1.672.1°C ± 2.333.2 h ± 2.731.5 ± 1.8
Adr/AN-9 (cell free)74.3°C ± 3.575.6°C ± 4.031.7 h ± 4.428.1 ± 1.7
Adr/AN-9 (cells)75.1°C ± 0.473.5°C ± 7.734.0 h ± 3.029.1 ± 2.9