Table 1

Thermal denaturation profile of Adriamycin adducts in relation to sequence specificity

PhosphorImage analysis of the gel in Fig. 5 enabled determination of Tms of individual blockage sites. The decreasing intensity of the band corresponding to each blockage site was plotted, and the temperature at which 50% of the intensity of the band remained was determined as the Tm. The 5-bp sequence shown is the sequence located 1 nucleotide before each group of blockages presented in Fig. 2B.

SiteFragment lengthSequenceTm
1120CGGCG68.8 ± 1.4
2128CTCCA69.1 ± 1.7
3134TACCA68.7 ± 3.6
4190TGAGG65.3 ± 2.4
5203CACGC63.7 ± 2.4
6224GTTGG63.6 ± 2.4
7241TCTCG61.6 ± 1.7
8273GACGT59.5 ± 1.7
9291CGAAG60.8 ± 1.5