Table 1

Adozelesin- and bizelesin-induced changes in p53 and p21 protein levels in HCT116 cells

Cells were treated for 4 h and incubated for an additional 24 h in drug-free medium. Proteins were detected by Western blotting and quantitated as described in “Materials and Methods.” p53 and p21 protein levels are expressed as fold increases over control. Data are from three individual experiments and are expressed (±SE).

Drug doseaAdozelesinBizelesin
p5310.1 ± 1.25.2 ± 3.6
2.5×15.7 ± 1.17.9 ± 0.3
12.5×19.6 ± 1.715.7 ± 4.5
p215.7 ± 1.15.9 ± 0.2
2.5×6.6 ± 0.313.4 ± 2.5
12.5×0.9 ± 0.117.3 ± 3.5
  • a The drug concentration is expressed as a multiple of the IC50.