Table 2

The reduced phosphorylation of protein kinases in H460 cells

No.Protein kinasesPhos. epitopeMolecular weight (Mr)Relative trace quantitya
ControlDox (1 μm)Serum free
1ERK1/2T202/T20442,0001.00.640.20
2MEK1/2S22141,0001.00.730.15
3PKCαS65776,0001.00.810.55
4PKCα/βT638/64176,0001.00.710.31
5SAPK/JNKT183/T18546,0001.00.380.30
6SAPK/JNKT183/T18539,0001.00.440.56
  • a Trace quantity: The trace quantity of a band is measured by the area under its intensity profile curve. Units are intensity × mm. The relative trace quantities were calculated based on the cells cultured in 10% FBS medium in which the level of phosphorylation of protein kinase was set as 1. The data is average of two separate experiments. The difference of data from the two separate experiments is <15% of the average values.