Table 1

Sensitivity of tumor cell lines to PXD101 and cisplatin

Drug sensitivity was determined by colony formation following drug exposure for 24 h. Results are the mean ± SEM of three experiments. Total HDAC activity (cpm/min/mg protein) was determined with cell lysates, and the results for two estimations are shown. HDAC inhibition was determined from a single dose response curve with duplicate points.

TumorCell lineTotal HDAC activityIC50 (nM) HDAC inhibitionIC50 (μM)
PXD101Cisplatin
OvaryA278039.2100.20 ± 0.050.08 ± 0.01
37.0
A2780/cp7024.7571.03 ± 0.172.00 ± 0.13
23.0
2780AD17.891.27 ± 0.191.23 ± 0.06
19.5
ColonHCT11611.8690.20 ± 0.010.99 ± 0.01
12.8
HT2920.7150.66 ± 0.052.43 ± 0.19
19.5
LungWIL8.2130.21 ± 0.030.79 ± 0.20
8.8
CALU-317.2110.66 ± 0.140.93 ± 0.14
17.6
BreastMCF723.6160.42 ± 0.221.05 ± 0.03
25.9
ProstatePC38.7260.23 ± 0.020.34 ± 0.01
11.0
MelanomaHS85210.81013.37 ± 1.440.81 ± 0.06
10.8