Table 2

Mutations in colcemid- and vinblastine-resistant cell lines

Cell lineWild-type sequence/mutant tubulin sequenceAmino acid change
Cmd4CAG-CTG-GAC-CGA-ATCβ D45Y
CAG-CTG-TAC-CGA-ATCa
CV 1-1TAT-GGG-GAC-CTC-AACβ D224N
CV 1-9TAT-GGG-AAC-CTC-AAC
CV 7-1GAC-ATC-TGC-TTC-CGCβ C211F
GAC-ATC-TTC-TTC-CGC
CV 4-4AAC-GTC-AAG-ACC-GCCβ K350N
AAC-GTC-AAT-ACC-GCC
CV 8-1ACC-ATG-AGC-GGG-GTCβ S234N
ACC-ATG-AAC-GGG-GTC
CV 1-2GCC-TAC-CAT-GAG-CAGα H283Y
CV 1-5, CV 1-7GCC-TAC-TAT-GAG-CAG
CV 2-8TTC-AGT-GAG-ACA-GGCα E55K
TTC-AGT-AAG-ACA-GGC
CV 2-3ACC-ACA-GCC-ATC-GCTα A383V
ACC-ACA-GTC-ATC-GCT
VV 3-2TGG-GCT-CGC-CTA-GATα R390C
TGG-GCT-TGC-CTA-GAT
  • a Nucleotides in bold represent the mutations found in each of the mutant cell lines.