Table 1

Real-time PCR primer and probe sequences

Primer
DHFR (fwd)5′-TAAACTGCATCGTCGCTGTGT-3′
DHFR (rev)5′-AGGTTGTGGTCATTCTCTGGAAA-3′
DHFR (probe)5′-6FAM-CCCGTTCTTGCCGATGCCCA-TAMRA-3′
RFC (fwd)5′-CCTCCTGGTGTCAGCAAGCT-3′
RFC (rev)5′-CCCGAGAGTCACTGGTTCACA-3′
RFC (probe)5′-6FAM-CCGTCGCTTGGAAGACACTGCAAA-TAMRA-3′
TS (fwd)5′-CTGTCCTGCCAGCTGTACCA-3′
TS (rev)5′-GCGTAGCTGGCGATGTTGA-3′
TS (probe)5′-6FAM-CCGAGGCCCATGTCTCCCGAT-TAMRA-3′
E2F-1 (fwd)5′-CGGTGTCGTCGACCTGAACT-3′
E2F-1 (rev)5′-AGGACGTTGGTGATGTCATAGATG-3′
E2F-1 (probe)5′-6FAM-CTGCACCTTCAGCACCTCGGCA-TAMRA-3′
E2F-4 (fwd)5′-CATCTGCTGTTTCTACACCTCCAC-3′
E2F-4 (rev)5′-CTATTTGGACGTGAGGCTTCCT-3′
E2F-4 (probe)5′-6FAM-AGCCTGCCCTAGCCCAGTCCCA-TAMRA-3′
β-Actin (fwd)5′-TGAGCGCGGCTACAGCTT-3′
β-Actin (rev)5′-TCCTTAATGTCACGCACGATTT-3′
β-Actin (probe)5′-6FAM-ACCACCACGGCCGAGCGG-TAMRA-3′