Table 1

Specific activities (μmol min−1 mg−1) of GST-catalyzed glutathionylation of CDNB and JS-K in 0.10 m phosphate buffer at pH 6.5 and 25°Ca

EnzymeCDNBJS-K
hGSTA1-1343.7
hGSTM1-120014.9
hGSTP1-1 (I104, A113)950.15
  • a The concentrations of CDNB and JS-K were 1.0 and 0.045 mm, respectively. The GSH concentration was 1.0 mm for both substrates. The protein concentrations were 0.025, 0.01, and 0.215 mg/ml for hGSTA1-1, hGSTM1-1, and hGSTP1-1, respectively.