Table 3.

Structurally diverse selective Src inhibitors inhibit anchorage-independent growth of HT-29 cells in soft agar

HT-29 cells (1200 cells/well) were seeded in soft agar along with the indicated inhibitors as described in “Materials and Methods. ” Plates were incubated for 2 weeks and stained overnight in a solution of 1 μg/ml iodonitrotetrazolium salt. Colonies were counted at ×4 magnification, and for each treatment group the total number of colonies in triplicate wells was calculated (variation was minimal between wells within groups).

ConditionsCompoundsConcentration (μm)Total no. of colonies% Inhibition
Untreated261
0.216437
SU113331.0399
2.00100
0.22504
High SerumSU113361.014146
2.00100
0.218728
CGP776751.013349
2.04782
Untreated81
0.24643
SU113331.0297
2.00100
Low Serum0.27310
SU113361.05828
2.00100
0.25433
CGP776751.04939
2.00100