Table 4

Amino acid sequence matching of GIP peptide P149 with various seven-transmembrane domain G-coupled cell surface receptors

Protein nameAmino acid sequence matching% Identity/similarity% Total
AFP peptide P149L S E D K L L A C G B G A A D D I I I G H L C I R H E M T P V N P G V G N100/0100
Fragments of P149∣←P149a→∣←P149b→∣←P149c→∣
1 Human TGF-βL S E D Q L L86/086
2 Mouse prolactin receptorL S E G K L L A86/086
3 Xenopus acetylcholine (δ) receptorL S G D K L L S75/1388
4 Human gastrin receptorL S A D K L L86/086
5 Mouse insulin growth factor-II receptorE A R L L A C71/1485
6 Drosophila EGF receptorA C G E G A E G N L L55/1873
7 Pig calcitoninI I I V H L83/083
8 Mouse T-cell receptor (BV)C I R D N K T P63/1376
9 Human dopamine receptorN P V D O G V G D66/2288
10 Human FKH proteinM T P V D P G V A Q70/30100
11 Mouse fibroblast growth factor receptorE M T P A N P G88/088
12 Rat somatostatin receptorT T P I A P G V63/2588
13 Human homeprotein FKHM T P V N P G V A Q80/20100
14 Rat glycine receptor tubulin linkerE M T P V L G T63/1376
15 Human T-cell CD2BN M T P R R P G63/1376
16 Mouse insulin growth factor-II receptorR M T P L T P P L44/3882
  • L S E D K L L, predicted internalization signal motif; consensus sequence X S/T X X R/K L L; P X X P, Src motif for protein-protein interaction; TGF, transforming growth factor; EGF, epidermal growth factor; FKH, forkhead.

  • The amino acid sequences of the GIP and its fragments (extending from 445 to 480) have been matched to various GPCRs and associated proteins using GCG-FASTA Software (see Fig. 2 legend). Most of the sequences were matched to the carboxyl terminus (P149c), fewer to the amino terminus (P149a), and the remainder fewest to the midpiece fragment (P149c).