Table 2

Comparison of apoptosis measured by PI staining and caspase-3 activation

% Apoptosis
ControlRituximab (20 μg/ml)Paclitaxel (10 nm)Rituximab + paclitaxel
PI staining1.32 ± 0.261.85 ± 0.812.08 ± 1.446.9 ± 1.9
Caspase-3 activation5.34 ± 1.829.7 ± 2.810.9 ± 12.246.7 ± 3.3
  • Note: The Ramos cells were treated under the same conditions explained in Table 1. Thereafter, the samples were divided in two equal proportions. One half was subjected to PI staining and the percent apoptosis was measured by the percent of hypodiploid cells accumulated at sub-G0 phase of the cell cycle. The second half was subjected to staining with FITC-labeled anti-active caspase-3 mAb and fluorescence-activated cell sorting analysis. Samples were set up in duplicates and the results are represented as means ± SEM (n = 2).