Table 5.

Kinetic properties of CPT-11 and BP-CPT by rCE and hiCE

EnzymeKmm)aSN-38 (pmol/h/mg CE protein)
CPT-11rCE6.218,963
BP-CPTrCE19.5813
hiCE0.65326
hCE1>50075
  • a Km values were calculated from hyperbolic plots using GraphPad Prism software.