Effect of AITC and BITC on HL60/S cell cycle progression
Note: Cells were grown in 10-cm plates for 24 h and then exposed to 10 μm AITC or BITC. The exposure lasted for either 24 h, or for 3 h (followed by growing the cells in ITC-free medium for 21 h). The cells then were harvested and analyzed for cell cycle distribution by flow cytometry. Each value is a mean of three measurements (±SD). Each value labeled with an asterisk is significantly different from the control (t test, P < 0.01).
ITC Exposure | ITC | Cell Cycle Distribution, % | ||||
---|---|---|---|---|---|---|
G1 | S | G2-M | ||||
3 h ITC + 21 h ITC-free | vehicle | 32.2 ± 2.9 | 51.1 ± 1.8 | 16.6 ± 2.0 | ||
AITC | 53.6 ± 18.6* | 33.1 ± 17.5 | 13.4 ± 1.3 | |||
BITC | 39.0 ± 13.0 | 15.9 ± 9.5 | 45.8 ± 20.1* | |||
24 h ITC | vehicle | 32.0 ± 0.5 | 57.9 ± 2.3 | 10.1 ± 2.6 | ||
AITC | 57.9 ± 6.0* | 38.5 ± 8.2 | 3.6 ± 2.4 | |||
BITC | 43.8 ± 1.1* | 37.2 ± 1.2 | 18.9 ± 1.4* |