Table 4.

Effect of AITC and BITC on HL60/S cell cycle progression

Note: Cells were grown in 10-cm plates for 24 h and then exposed to 10 μm AITC or BITC. The exposure lasted for either 24 h, or for 3 h (followed by growing the cells in ITC-free medium for 21 h). The cells then were harvested and analyzed for cell cycle distribution by flow cytometry. Each value is a mean of three measurements (±SD). Each value labeled with an asterisk is significantly different from the control (t test, P < 0.01).

ITC Exposure	ITC	Cell Cycle Distribution, %
ITC Exposure	ITC			G₁	S	G₂-M
3 h ITC + 21 h ITC-free	vehicle	32.2 ± 2.9	51.1 ± 1.8	16.6 ± 2.0
		AITC	53.6 ± 18.6*	33.1 ± 17.5	13.4 ± 1.3
		BITC	39.0 ± 13.0	15.9 ± 9.5	45.8 ± 20.1*
24 h ITC	vehicle	32.0 ± 0.5	57.9 ± 2.3	10.1 ± 2.6
		AITC	57.9 ± 6.0*	38.5 ± 8.2	3.6 ± 2.4
		BITC	43.8 ± 1.1*	37.2 ± 1.2	18.9 ± 1.4*