Table 2

Expression of XAGE-1 in human cancer cell lines

Either total or poly(A) RNA was isolated from the tumor cell lines. Expression levels were determined by RT-PCR using Xa-1 and Xa-2 primers to the XAGE-1 gene (see Fig. 1A). These primers are located in different exons and amplify a 273-bp product. Separate PCR reactions were performed using actin primers to verify the quality of the generated cDNA.

Cell lineCancer typeLevel of XAGE-1 expression
LNCaPProstateStrong
PC3ProstateNone
DU145ProstateStrong
ZR-75-1BreastNone
MCF7BreastNone
BT-474BreastNone
MDA-MB-231BreastStrong
SK-BR-3BreastWeak
MDA-MB-468BreastWeak
OVCAROvarianStrong
FEM-XMelanomaWeak
HUT102T cell lymphomaWeak
U937Histiocytic lymphomaStrong
DaudiBurkitt’s lymphomaNone
JD38Burkitt’s lymphomaNone
RajiBurkitt’s lymphomaNone
A-172GlioblastomaWeak
IMR-32NeuroblastomaNone
Colo 205ColonNone
LOVOColonNone
SW403RectumNone
SW480Colorectal adenocarcinomaNone
SW620Colorectal adenocarcinomaNone