Table 1

Expression of XAGE-1 in lung cancers

Total RNA was isolated from frozen tumor samples, and expression levels were determined by RT-PCR using primers Xa-1 and Xa-2 to the XAGE-1 gene (see Fig. 1A). These primers are located in different exons and amplify a 273-bp product. The RNA templates were checked for integrity using the Agilent 2100 Bioanalyzer, and separate PCR reactions were performed using actin primers to confirm the quality of the generated cDNA.

Lung cancer typeTotal no. testedNo. of cancers that express XAGE-1% of samples expressing XAGE-1
SCLC33100
NSCLC22100
Squamous cell carcinoma3267
Adenocarcinoma3267