Table 3.

Cytotoxicity of FTC analogues

Values are means of quadruplicate repeats. SDs in no case exceeded 25% of the means and have therefore been omitted for clarity. In an independent repetition of the experiments, only one result differed by >30% from the value shown; it is shown in parentheses. The origins and relevant properties of the cell lines are detailed in “Materials and Methods” and “Results.”

Cell lineTypeIC50m)
Ko132Ko134Ko143GF120918
Human
 2008Ovarian13172517
 2008/MRP1Transfected: high MRP117182620
 IGROV1Ovarian2622341.3 (0.65)
 IGROV1/T8Topotecan-selected: high BCRP2226271.0
 A549Lung159.0254.0
 CCRF/CEMT-lymphoblast2022242.7
 MCF-7Mammary15151918
 WiDrColon1813281.1
Mouse fibroblast
 NIH3T3Wild-type1318270.85
 2ac.1Wild-type19193017
 77.1Mdr1a/1b−/−14163030
 77.1/MDR1Transduced: high P-gp29212518
 MEF3.8Mdr1a/1b−/−Mrp1−/−1415251.4
 MEF3.8/T6400Topotecan-selected: high Bcrp11211.5181.2