Table 3.

MAD2 expression

The human glioma cell line GB2 was treated with CP248, CP461, or exisulind at the respective IC50 concentrations for 18 h and immunostained for MAD2, as described in “Materials and Methods.” MAD2 overexpression was defined as the percentage of mitotic cells in which >50% of the chromosomes stained positive for MAD2. An example is shown in Fig. 2C. Mitotic cells on a single glass coverslip were scored for MAD2 expression by microscopy with a ×100 Nikon objective. More than 150 mitotic cells were scored for each of the treatment conditions.

DrugGB2 Mad2 overexpression
(%)(SD)
No drug180
Colcemid982.4
CP248585.7
CP461201.4
Exisulind181.4