Table 2.

Flow cytometric analysis

Cell cycle analysis was performed as described in “Materials and Methods.” Cells were treated with the indicated compounds at their respective IC50 values (see Table 1), collected at the indicated time points, and analyzed by DNA flow cytometry. The values obtained are an average of two independent experiments, with a minimum of 15,000 events collected for each time point. Duplicates agreed within 5%.

Treatment
6 h (%)
12 h (%)
24 h (%)
9L cell line
No treatment
 G0-G146.148.452.7
 S32.736.132.0
G219.313.813.8
M1.81.81.6
 >G2-M3.92.93.5
CP248 (0.078 μm)
 G0-G127.231.832.8
 S35.228.442.4
G224.524.220.5
M13.115.74.4
 >G2-M4.07.017.4
CP461 (1.14 μm)
 G0-G144.444.655.4
 S33.536.930.0
G219.715.912.7
M2.42.71.9
 >G2-M2.62.72.3
Exisulind (180 μm)
 G0-G148.850.758.4
 S30.234.528.8
G219.513.211.5
M1.61.71.3
 >G2-M2.12.32.4
GB1 cell line
No treatment
 G0-G147.554.738.0
 S17.813.930.9
G232.729.228.7
M2.02.22.4
 >G2-M8.76.49.4
CP248 (0.07 μm)
 G0-G144.142.521.1
 S18.717.330.4
G232.930.332.1
M4.99.916.4
 >G2-M8.58.811.7
CP461 (0.8 μm)
 G0-G148.754.238.1
 S19.014.730.2
G229.127.528.3
M2.33.73.4
 >G2-M7.56.59.7
Exisulind (24 μm)
 G0-G147.953.749.0
 S19.214.124.8
G231.229.824.3
M1.82.42.0
 >G2-M7.87.47.1
Colcemid (0.13 nm)
 G0-G141.642.411.8
 S19.713.842.0
G237.138.435.8
M1.73.910.4
 >G2-M9.910.113.6