Table 4

Cell cycle effectsa and induction of apoptosisb by GW2016c

Cell linePhaseVehicle control1 μm GW201610 μm GW2016
BT474G1507028
S3712
G2/M988
Apoptosis (Sub-2N DNA)11857
HN5G1519179
S3921
G2/M849
Apoptosis (Sub-2N DNA)118
  • a Values were determined by gate analysis of flow cytometric plots in Fig. 5 and are presented as a percentage of the total cell population, after eliminating cell doublets. The addition of values for each treatment do not total 100% attributable to the presence of a small population of events appearing outside of the normal cell cycle.

  • b Apoptotic events are inferred by the presence of cells with <2N DNA.

  • c Cells were treated for 72 h, followed by flow cytometric analysis.