Table 3

The IC50 valuesam) for inhibition of cell growth by 72-h treatment with GW2016, Iressa, or OSI-774

Cell lineRelative expressionb
GW2016ZD1839OSI-774
EGFRErbB-2
HFF+c12 ± 1.4>10>8.7
MCF-7++4.0 ± 0.114.2 ± 0.6>30
T47D++3.0 ± 0.210.5 ± 0.514.5 ± 0.6
A-431++++0.16 ± 0.030.08 ± 0.020.10 ± 0.03
HN5++++0.12 ± 0.030.08 ± 0.010.18 ± 0.02
BT474++++0.10 ± 0.031.1 ± 0.19.9 ± 1.6
N87++++0.09 ± 0.022.6 ± 0.94.6 ± 0.5
CaLu-3ND+++0.13 ± 0.03NDND
HB4a+9.1 ± 0.814.5 ± 0.423.8 ± 2.1
HB4a c5.2++++0.21 ± 0.031.1 ± 0.41.8 ± 0.7
  • a The IC50 values are averages of a minimum of two experiments and are presented with the SE.

  • b Relative EGFR and ErbB-2 protein expression of each cell line was confirmed by Western blot analysis or, in the case of A431, inferred from the literature (41).

  • c −, below detectable levels by Western blot; ND, not determined.