Table 2

The IC50 values for inhibition of receptor tyrosine phosphorylationa

Cell lineImmunoprecipitated receptorbPhosphotyrosine inhibitiona (IC50 μm)
BT474EGFR0.17 ± 0.03
ErbB-20.08 ± 0.02
HN5EGFR0.21 ± 0.06
0.06 ± 0.03
  • a Cells were treated for 6 h with GW2016.

  • b Receptors were immunoprecipitated and subjected to western blot analysis for phosphotyrosine content as described in “Materials and Methods.”

  • c Relative phosphotyrosine content was quantified by laser densitometry from five or six point dose-response curves. Average IC50 values from four individual experiments are presented with the SE.