Table 2

Affinity of SS dsFv and (dsFv)2 immunotoxins

The affinity of SS dsFv and (dsFv)2 immunotoxins was determined by surface plasmon resonance (BiaCore). Kass, kdiss, and KD (KD = kdiss/kass; binding at equilibrium) were calculated from the sensorgrams using the BIA evaluation software package.




AffinitySS dsFv(dsFv)2
kass (m−1 s−1)5.21 × 1051.16 × 106
kdiss (s−1)5.49 × 10−33.13 × 10−4
KD (m)
10.5 × 10−9
2.6 × 10−10